LRRFIP2 Antibody

• Background:

  LRRFIP2 Antibody: The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI. It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of beta-catenin. LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-κ B during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.

• NCBI Gene ID:

  9209

• Swiss Prot:

  Q9Y608

• Accession Number:

  NP_006300

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Innate Immunity

• Purification:

  LRRFIP2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1472 - Rat Colon Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  LRRFIP2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LRRFIP2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.

• Symbol:

  LRRFIP2

• NCBI Official Name:

  Leucine rich repeat (in FLII) interacting protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Fong KS and de Couet HG. Novel proteins interacting with the leucine-rich repeat domain of human flightless-I identified by the yeast two-hybrid system. Genomics 1999; 58:146-57.

• Background Reference 02:

  Liu J, Bang AG, Kintner C, et al. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis. Proc. Natl. Acad. Sci. USA 2005; 102:1927-32.

• Background Reference 03:

  Dai P, Jeong SY, Yu Y, et al. Modulation of TLR signaling by multiple MyD88-interacting including leucine-rich repeat Fli-I-interacting proteins. J. Immunol. 2009; 182:3450-60.

• Background Reference 04:

  Gunawardena HP, Huang Y, Kenjale R, et al. Unambiguous characterization of site-specific phosphorylation of Leucine-rich FLI-I-interacting protein 2 (LRRFIP2) in Toll-like receptor 4 (TLR4) -mediated signaling. J. Biol. Chem. 2011; epub.

• Other Product Names:

  LRRFIP2 Antibody: HUFI-2, Leucine-rich repeat flightless-interacting protein 2, LRR FLII-interacting protein 2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  5453726

• Physical Properties:

  Liquid