GLS2 Antibody

• Background:

  GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

• NCBI Gene ID:

  27165

• Swiss Prot:

  Q9UI32

• Accession Number:

  NP_037399

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  GLS2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1464 - Rat Liver Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  GLS2 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Multiple isoforms of GLS2 are known to exist.

• Symbol:

  GLS2

• NCBI Official Name:

  Glutaminase 2 (liver, mitochondrial)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200.

• Background Reference 02:

  Gomez-Fabre PM, Aledo JC, del Castillo-Olivares A, et al. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 2000; 345:365-75.

• Background Reference 03:

  Hu W, Zhang C, Wu R, et al. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. USA 2010; 107:7455-60.

• Other Product Names:

  GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase liver isoform, mitochondrial, L-glutaminase

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  20336214

• Physical Properties:

  Liquid