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CRISP1 Antibody

CAT: 0223-30-887Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-30-887Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. CRISP1 is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface.Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.
NCBI Gene ID
167
Swiss Prot
P54107
Accession Number
NP_001122
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRISP1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
721_B Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CRISP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
27 kDa
Applications Notes
CRISP1 antibody can be used for detection of CRISP1 by ELISA at 1:312500. CRISP1 antibody can be used for detection of CRISP1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
CRISP1
NCBI Official Name
Cysteine-rich secretory protein 1
NCBI Organism
Homo sapiens
Background Reference 01
Mungall, A.J., (2003) Nature 425 (6960), 805-811.
Other Product Names
CRISP1, AEGL1, ARP, CRISP-1, HSCRISP1D, HSCRISP1G, HUMARP
Tested Applications
ELISA, WB
Protein ID
25121982
Physical Properties
Liquid

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