MAK10 Antibody

• Background:

  MAK10 Antibody: The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.

• NCBI Gene ID:

  60560

• Swiss Prot:

  Q5VZE5

• Accession Number:

  NP_078911

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  MAK10 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1401 - Mouse Heart Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken: (100%)

• Buffer:

  MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  MAK10 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  MAK10

• Positive Control 2:

  Cat. No. 10-501 - Human Heart Tissue Slide

• NCBI Official Name:

  N (alpha) -acetyltransferase 35, NatC auxiliary subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lee YJ et al. MAK10, a glucose repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs. Genetics1992; 132:87-96.

• Background Reference 02:

  Yi XJ, et al. A novel epithelial wound-related gene is abundantly expressed in developing rat cornea and skin. Curr. Eye Res.2000; 20:430-40.

• Background Reference 03:

  Wenzlau JM, et al. Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development. Circ. Res.2006; 98:846-55.

• Background Reference 04:

  Weiser-Evans MC, et al. Novel embryonic genes are preferentially expressed by autonomously replicating rat embryonic and neointimal smooth muscle cells. Circ. Res.2000; 87:608-15.

• Other Product Names:

  MAK10 Antibody: EGAP, MAK10, MAK10P, bA379P1.1, EGAP, N-alpha-acetyltransferase 35, NatC auxiliary subunit, Embryonic growth-associated protein homolog

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  145275204

• Physical Properties:

  Liquid