RSPO1 Antibody

• Background:

  RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

• NCBI Gene ID:

  284654

• Swiss Prot:

  Q2MKA7

• Accession Number:

  NP_001033722

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  5171

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Test (Figure 2) : Anti-RSPO1 antibodies (5171) detected human RSPO1 recombinant protein at different concentrations. Recombinant Protein Test5171

• Field of Research:

  Signal Transduction

• Purification:

  RSPO1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224-Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat (100%) .

• Buffer:

  RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  RSPO1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

• Isoform:

  Human RSPO1 has 3 isoforms, including isoform 1 (263aa, 29kD), isoform 2 (236aa, 26kD), and isoform 3 (200aa, 22kD) . Mouse RSPO1 had 1 isoform (265aa, 29kD) and rat RSPO1 had 1 isoform (262aa, 29kD) . 5171 can detect human, mouse and rat.

• Symbol:

  RSPO1

• Positive Control 2:

  Cat. No. 1301 - Human Heart Tissue Lysate

• Positive Control 3:

  Cat. No. 10-501 - Human Heart Tissue Slide

• Positive Control 4:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  R-spondin homolog (Xenopus laevis)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kamata et al. Biochim. Biophys. Acta 2004; 1676:51-61.

• Background Reference 02:

  Kim et al. Cell Cycle 2006; 5:23-6.

• Background Reference 03:

  Parma et al. Nat. Genet. 2006; 38:1304-9.

• Background Reference 04:

  Chassot et al. Hum. Mol. Genet. 2008; 17:1278-91.

• Other Product Names:

  RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof plate-specific spondin-1, hRspo1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  84490388

• Physical Properties:

  Liquid