Bora Antibody

• Background:

  Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

• NCBI Gene ID:

  79866

• Swiss Prot:

  Q6PGQ7

• Accession Number:

  Q6PGQ7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Bora Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  Bora Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Bora antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  BORA

• NCBI Official Name:

  Bora, aurora kinase A activator

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol.2002; 12:640-647.

• Background Reference 02:

  Wiese C and O'Brien LL. What's so Bor (a) ing about Aurora A activation? Dev. Cell2006; 11:133-134.

• Background Reference 03:

  Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell2006; 11:147-157.

• Background Reference 04:

  Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res.2007; 5:1-10.

• Other Product Names:

  Bora Antibody: C13orf34, C13orf34, Protein aurora borealis, HsBora

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  74737659

• Physical Properties:

  Liquid