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ATG5 Antibody

CAT: 0223-5031-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-5031-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3) -like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
NCBI Gene ID
9474
Swiss Prot
A9UGY9
Accession Number
EAW48415
Host
Chicken
Reactivity
Human, Mouse
Immunogen
5031
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar ATG5 expression profile in human and mouse cell lines detected by two independent anti-ATG5 antibodies that recognize different epitopes, 5031 against N-terminus domain and 64-229 against human recombinant protein. ATG5 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5031
Field of Research
Autophagy
Purification
ATG5 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat (100%), Bovine: (94%), Pig: (94%)
Buffer
ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
ATG5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgY
Specificity
Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
Isoform
Human ATG5 has 2 isoforms, including isoform L (275aa, 32.4kD) and isoform S (197aa, 22.9kD. This antibody detects only human isoform L. Mouse ATG5 has 1 isoform (275aa, 32.4kD) . Rat ATG5 has only one isoform identified so far (275aa, 32.4kD) .
Symbol
ATG5
Positive Control 2
Cat. No. 10-901 - Human Spleen Tissue Slide
NCBI Official Name
ATG5 autophagy related 5 homolog (S. cerevisiae)
NCBI Organism
Homo sapiens
Background Reference 01
Gozuacik and Kimchi. Oncogene 2004; 23:2891-906.
Background Reference 02
Kisen et al. Carcinogenesis 1993; 14:2501-5.
Background Reference 03
Kamada et al. J. Cell. Biol. 2000; 150:1507-13.
Background Reference 04
Hanada et al. J. Biol. Chem.2007; 282:37298-302.
Other Product Names
ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
Tested Applications
ELISA, WB, IHC-P
Protein ID
119568800
Physical Properties
Liquid

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