EBI3 Antibody

• Background:

  EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

• NCBI Gene ID:

  10148

• Swiss Prot:

  Q14213

• Accession Number:

  NP_005746

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  4977

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Test (Figure 2) shows that anti-IL-EBI-3 antibody (4977) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4977 antigenic peptide. Recombinant Protein Test

• Field of Research:

  Immunology

• Purification:

  EBI3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1466 - Rat Spleen Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  EBI3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human EBI-3 has only 1 isoform (229aa, 25kD) . Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD) . 4977 can detect human isoform and possibly detect mouse and rat EBI-3.

• Symbol:

  EBI3

• Positive Control 2:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Epstein-Barr virus induced 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pfanz et al. Immunity2002; 16:779-90.

• Background Reference 02:

  Devergne et al. Proc. Natl. Acad. Sci. USA1997; 94:12041-6.

• Background Reference 03:

  Niedbala et al. Eur. J. Immunol.2007; 3021-29.

• Background Reference 04:

  Pfanz et al. J. Immunol.2004; 172:2225-31.

• Other Product Names:

  EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  14577917

• Physical Properties:

  Liquid