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EBI3 Antibody

CAT: 0223-4977-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-4977-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
NCBI Gene ID
10148
Swiss Prot
Q14213
Accession Number
NP_005746
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
4977
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Recombinant Protein Test (Figure 2) shows that anti-IL-EBI-3 antibody (4977) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4977 antigenic peptide. Recombinant Protein Test
Field of Research
Immunology
Purification
EBI3 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1466 - Rat Spleen Tissue Lysate
Concentration
1 mg/mL
Buffer
EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
EBI3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human EBI-3 has only 1 isoform (229aa, 25kD) . Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD) . 4977 can detect human isoform and possibly detect mouse and rat EBI-3.
Symbol
EBI3
Positive Control 2
Cat. No. 10-901 - Human Spleen Tissue Slide
NCBI Official Name
Epstein-Barr virus induced 3
NCBI Organism
Homo sapiens
Background Reference 01
Pfanz et al. Immunity2002; 16:779-90.
Background Reference 02
Devergne et al. Proc. Natl. Acad. Sci. USA1997; 94:12041-6.
Background Reference 03
Niedbala et al. Eur. J. Immunol.2007; 3021-29.
Background Reference 04
Pfanz et al. J. Immunol.2004; 172:2225-31.
Other Product Names
EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
14577917
Physical Properties
Liquid

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