LASS5 Antibody

• Background:

  LASS5 Antibody: The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

• NCBI Gene ID:

  91012

• Swiss Prot:

  Q8N5B7

• Accession Number:

  NP_671723

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  LASS5 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1220 - SK-N-SH Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  LASS5 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LASS5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Multiple isoforms of LASS5 are known to exist. This antibody is predicted not to cross-react with the highly homologous LASS6.

• Symbol:

  CERS5

• NCBI Official Name:

  Ceramide synthase 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem.2003; 278:43452-9.

• Background Reference 02:

  Lahiri S and Futerman AH. LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor. J. Biol Chem.2005; 280:33735-8.

• Background Reference 03:

  Nishi M, Sakagami H, Komazaki S, et al. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res.2003; 118:102-10.

• Background Reference 04:

  Xu Z, Zhou J, McCoy DM, et al. LASS5 is the predominant ceramide synthase isoform involved in de novo sphingolipid synthesis in lung epithelia. J. Lipid Res.2005; 46:1229-38.

• Other Product Names:

  LASS5 Antibody: Trh4, LASS5, Ceramide synthase 5, LAG1 longevity assurance homolog 5, CerS5

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  22218345

• Physical Properties:

  Liquid