SCRAPPER Antibody

• Background:

  SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

• NCBI Gene ID:

  84961

• Swiss Prot:

  Q96IG2

• Accession Number:

  NP_116264

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  SCRAPPER Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1288 - A20 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SCRAPPER antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  FBXL20

• Positive Control 2:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  F-box and leucine-rich repeat protein 20

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ho MS, Tsai PI, and Chien CT. F-box proteins: the key to protein degradation. J. Biomed. Sci.2006; 13:181-91.

• Background Reference 02:

  Dobie F and Craig AM. A fight for neurotransmission: SCRAPPER trashes RIM. Cell2007; 130:775-7.

• Background Reference 03:

  Yao I, Takagi H, Ageta H, et al. SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Cell2007; 130:943-57.

• Background Reference 04:

  Ding M, Chao D, Wang G, et al. Spatial regulation of an E3 ubiquitin ligase directs selective synapse elimination. Science2007; 317:947-51.

• Other Product Names:

  SCRAPPER Antibody: Fbl2, Fbl20, FBL2, F-box and leucine-rich repeat protein 20

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  27734755

• Physical Properties:

  Liquid