TEM5 Antibody

• Background:

  TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

• NCBI Gene ID:

  25960

• Swiss Prot:

  Q96PE1

• Accession Number:

  Q96PE1

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  TEM5 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1310 - Human Bladder Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (86%)

• Buffer:

  TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TEM5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  GPR124

• NCBI Official Name:

  G protein-coupled receptor 124

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.

• Background Reference 02:

  Yamamoto Y, Irie K, Nanda A, et al. Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein. Oncogene2004; 23:3889-97.

• Background Reference 03:

  Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.

• Background Reference 04:

  Vallon M and Essler M. Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha (v) beta3 to glycosaminoglycans. J. Biol. Chem.2006; 281:34179-88.

• Other Product Names:

  TEM5 Antibody: TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  221222450

• Physical Properties:

  Liquid