TEM1 Antibody

CAT: 0223-4357-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-4357-01Size:0.02 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
NCBI Gene ID
57124
Swiss Prot
Q9HCU0
Accession Number
NP_065137
Host
Rabbit
Reactivity
Human
Immunogen
4357
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain. TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4357 4359
Field of Research
Homeostasis, Cancer
Purification
TEM1 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1320 - Human Colon Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Mouse (86%) ; Rat: (86%)
Buffer
TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
TEM1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
Isoform
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD) . Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD) . 4357 can detect all isoforms of human, mouse and rat.
Symbol
CD248
Positive Control 2
Cat. No. 1223 - Caco-2 Cell Lysate
Positive Control 3
Cat. No. 1219 – MCF-7 Cell Lysate
Positive Control 4
Cat. No. 1220 – SK-N-SH Cell Lysate
NCBI Official Name
CD248 molecule, endosialin
NCBI Organism
Homo sapiens
Background Reference 01
Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.
Background Reference 02
Christian et al. J. Biol. Chem. 2001; 276:7408-14.
Background Reference 03
Nanda et al. Curr. Opin. Oncol. 2004; 16:44-9.
Background Reference 04
Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.
Other Product Names
TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
9966885
Physical Properties
Liquid

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