RAP80 Antibody

• Background:

  RAP80 Antibody: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR) . Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

• NCBI Gene ID:

  51720

• Swiss Prot:

  Q96RL1

• Accession Number:

  EAW85043

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  RAP80 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - HEK293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (100%), Rat: (88%)

• Buffer:

  RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  RAP80 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  UIMC1

• Positive Control 2:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Ubiquitin interaction motif containing 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yan Z, Kim YS, and Jetten AM. RAP80, a novel nuclear protein that interacts with the retinoid-related testis-associated receptor. J. Biol. Chem.2002; 277:32379-88.

• Background Reference 02:

  Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science2007; 316:1194-1198.

• Background Reference 03:

  Kim H, Huang J, and Chen J. CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response. Nat. Struct. Mol. Biol.2007; 14:710-5.

• Background Reference 04:

  Liu Z, Wu J, and Yu X. CCDC98 targets BRCA1 to DNA damage sites. Nat. Struct. Mol. Biol.2007; 14:716-20.

• Other Product Names:

  RAP80 Antibody: RAP80, X2HRIP110, RAP80, RXRIP110, BRCA1-A complex subunit RAP80, Receptor-associated protein 80

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  119605449

• Physical Properties:

  Liquid