TBC1D1 Antibody

• Background:

  TBC1D1 Antibody: TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.

• NCBI Gene ID:

  23216

• Swiss Prot:

  Q86TI0

• Accession Number:

  NP_055988

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis, Signal Transduction, Cancer

• Purification:

  TBC1D1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 – 293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (71%)

• Buffer:

  TBC1D1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TBC1D1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  TBC1D1

• NCBI Official Name:

  TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5.

• Background Reference 02:

  Koumanov F and Holman GD. Thrifty Tbc1d1 and Tbc1d4 proteins link signalling and membrane trafficking pathways. Biochem. J.2007; 403:e9-11.

• Background Reference 03:

  Roach WG, Chavez JA, Miinea CP, et al. Substrate specificity and effect on GLUT4 translocation of the Rab GTPase-activating protein Tbc1d1. Biochem J.2007; 403:353-8.

• Background Reference 04:

  Stone S, Abkevich V, Russell DL, et al. TBC1D1 is a candidate for a severe obesity gene and evidence for a gene/gene interaction in obesity predisposition. Hum. Mol. Genet.2006; 15:2709-20.

• Other Product Names:

  TBC1D1 Antibody: TBC, TBC1, KIAA1108, TBC1 domain family member 1

• Tested Applications:

  ELISA, WB

• Protein ID:

  50658061

• Physical Properties:

  Liquid