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Bub3 Antibody

CAT: 0223-4227-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-4227-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
NCBI Gene ID
9184
Swiss Prot
O43684
Accession Number
O43684
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Homeostasis, Signal Transduction
Purification
Bub3 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Buffer
Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Bub3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Symbol
BUB3
Positive Control 2
Cat. No. 17-005 - Jurkat Cell Slide
NCBI Official Name
Budding uninhibited by benzimidazoles 3 homolog (yeast)
NCBI Organism
Homo sapiens
Background Reference 01
Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev.2000; 14:2277-82.
Background Reference 02
Taylor SS, Ha E and McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol.1998; 142:1-11.
Background Reference 03
Warren CD, Brady DM, Johnston RC, et al. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol. Biol. Cell2002; 13: 3029-41.
Background Reference 04
Roberts BT, Farr KA and Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol.1994; 14:8282-91.
Other Product Names
Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint protein BUB3
Tested Applications
ELISA, WB, ICC, IF
Protein ID
7387554
Physical Properties
Liquid

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