Bub3 Antibody

• Background:

  Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

• NCBI Gene ID:

  9184

• Swiss Prot:

  O43684

• Accession Number:

  O43684

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis, Signal Transduction

• Purification:

  Bub3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Bub3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  BUB3

• Positive Control 2:

  Cat. No. 17-005 - Jurkat Cell Slide

• NCBI Official Name:

  Budding uninhibited by benzimidazoles 3 homolog (yeast)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev.2000; 14:2277-82.

• Background Reference 02:

  Taylor SS, Ha E and McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol.1998; 142:1-11.

• Background Reference 03:

  Warren CD, Brady DM, Johnston RC, et al. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol. Biol. Cell2002; 13: 3029-41.

• Background Reference 04:

  Roberts BT, Farr KA and Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol.1994; 14:8282-91.

• Other Product Names:

  Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint protein BUB3

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  7387554

• Physical Properties:

  Liquid