DARC Antibody

• Background:

  DARC Antibody: DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.

• NCBI Gene ID:

  2532

• Swiss Prot:

  Q16570

• Accession Number:

  Q16570

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  DARC Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1403 - Mouse Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  DARC Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DARC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  DARC

• NCBI Official Name:

  Duffy blood group, chemokine receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chaudhuri A, Polyakova J, Zbrzezna V, et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA1993; 90:10793-7.

• Background Reference 02:

  Gardner L, Patterson AM, Ashton BA, et al. The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun.2004; 321:306-12.

• Background Reference 03:

  Gil ML, Vita N, Lebel-Binay S, et al. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol.1992; 2826-33.

• Other Product Names:

  DARC Antibody: FY, Dfy, GPD, GpFy, ACKR1, CCBP1, CD234, WBCQ1, FY, Atypical chemokine receptor 1

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  67476970

• Physical Properties:

  Liquid