PD-1 Antibody

• Background:

  PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

• NCBI Gene ID:

  5133

• Swiss Prot:

  Q15116

• Accession Number:

  Q15116

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  4065

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 1) : Anti-PD-1 antibodies (4065) specificity was further verified by PD-1 specific knockout. PD-1 signal was not detected in PD-1 knockout HeLa cells as compared to that in control wild type cells. KO Validation4065) KD validation (Figure 2) : Anti-PD-1 antibody (4065) specificity was further verified by PD-1 specific knockdown. PD-1 signal in HeLa cells transfected with PD-1 siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs. KD validation4065

• Field of Research:

  Apoptosis

• Purification:

  PD-1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

• Fragment:

  IgG

• Isoform:

  Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

• Symbol:

  PDCD1

• Positive Control 2:

  Cat. No. 1205 - Jurkat Cell Lysate

• Positive Control 3:

  Cat. No. 1208 - THP-1 Cell Lysate

• Positive Control 4:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  Programmed cell death 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holling et al. Hum. Immunol. 2004; 65:282-90.

• Background Reference 02:

  Ishida et al. EMBOJ. 1992;11:3887-95.

• Background Reference 03:

  Zhong et al. Int. Immunol. 2004; 16:1181-8

• Background Reference 04:

  Nishimura et al. Immunity 1999;11:141-51

• Other Product Names:

  PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  145559515

• Physical Properties:

  Liquid