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PD-1 Antibody

CAT: 0223-4065-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-4065-01Size:0.02 mg
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Background
PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.
NCBI Gene ID
5133
Swiss Prot
Q15116
Accession Number
Q15116
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
4065
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KO Validation (Figure 1) : Anti-PD-1 antibodies (4065) specificity was further verified by PD-1 specific knockout. PD-1 signal was not detected in PD-1 knockout HeLa cells as compared to that in control wild type cells. KO Validation4065) KD validation (Figure 2) : Anti-PD-1 antibody (4065) specificity was further verified by PD-1 specific knockdown. PD-1 signal in HeLa cells transfected with PD-1 siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs. KD validation4065
Field of Research
Apoptosis
Purification
PD-1 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1204 - K562 Cell Lysate
Concentration
1 mg/mL
Buffer
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PD-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.
Fragment
IgG
Isoform
Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.
Symbol
PDCD1
Positive Control 2
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 4
Cat. No. 10-301 - Human Brain Tissue Slide
NCBI Official Name
Programmed cell death 1
NCBI Organism
Homo sapiens
Background Reference 01
Holling et al. Hum. Immunol. 2004; 65:282-90.
Background Reference 02
Ishida et al. EMBOJ. 1992;11:3887-95.
Background Reference 03
Zhong et al. Int. Immunol. 2004; 16:1181-8
Background Reference 04
Nishimura et al. Immunity 1999;11:141-51
Other Product Names
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
145559515
Physical Properties
Liquid

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