DRAM Antibody

• Background:

  DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

• NCBI Gene ID:

  55332

• Swiss Prot:

  Q8N682

• Accession Number:

  AAH18435

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  4035

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar DRAM expression profile in human, mouse and rat cell lines detected by two independent anti-DRAM antibodies that recognize different epitopes, 4033 against C-terminus domain and 4035 against N-terminus domain. DRAM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4033 4035

• Field of Research:

  Apoptosis, Autophagy, Cancer

• Purification:

  DRAM Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  DRAM Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DRAM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human DRAM has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (128aa, 14kD) . Mouse DRAM also has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (132aa, 15kD) . Rat DRAM has only one isoform (238aa, 26kD) . 4035 can detect both human isoforms , and also detect mouse and rat.

• Symbol:

  DRAM1

• Positive Control 2:

  Cat. No. 1304 - Human Liver Tissue Lysate

• Positive Control 3:

  Cat. No. 10-201 - Human Liver Tissue Slide

• NCBI Official Name:

  Damage-regulated autophagy modulator

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Crighton D, Wilkinson S, O'Prey J, et al. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006; 126:121-34.

• Background Reference 02:

  Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.

• Background Reference 03:

  Crighton D, Wilkinson S, and Ryan KM. DRAM links autophagy to p53 and programmed cell death. Autophagy 2007; 3:72-4.

• Other Product Names:

  DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  22450862

• Physical Properties:

  Liquid