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DRAM Antibody

CAT: 0223-4033-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#: 4033-01Size: 0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
NCBI Gene ID
55332
Swiss Prot
Q8N682
Accession Number
AAH18435
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
4033
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar DRAM expression profile in human, mouse and rat cell lines detected by two independent anti-DRAM antibodies that recognize different epitopes, 4033 against C-terminus domain and 4035 against N-terminus domain. DRAM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4033 4035 Induced Expression Validation (Figure 5) : DRAM expression detected by anit-DRAM antibodies (4033) was up-regulated by CQ treatment in WT neural precursor cells. Induced Expression Validation4033 Regulated expression validation (Figure 6) : DRAM expression detected by anit-DRAM antibodies (4033) was down-regulated by in WT neural precursor cells. Regulated expression validation4033
Field of Research
Apoptosis, Autophagy, Cancer
Purification
DRAM Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1210 - HEK293 Cell Lysate
Concentration
1 mg/mL
Buffer
DRAM Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
DRAM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human DRAM has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (128aa, 14kD) . Mouse DRAM also has 2 isoforms, including isoform 1 (238aa, 26kD) and isoform 2 (132aa, 15kD) . Rat DRAM has only one isoform (238aa, 26kD) . 4033 can detect human, mouse and rat isoforms.
Symbol
DRAM1
Positive Control 2
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 3
Cat. No. 10-201 - Human Liver Tissue Slide
NCBI Official Name
Damage-regulated autophagy modulator
NCBI Organism
Homo sapiens
Background Reference 01
Crighton, et al. Cell 2006; 126:121-34.
Background Reference 02
Gozuacik and Kimchi. Oncogene. 2004; 23:2891-906.
Background Reference 03
Crighton, et al. Autophagy 2007; 3:72-4.
Citation 01
20123985
Citation 02
21720543
Other Product Names
DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
22450862
Physical Properties
Liquid

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