TMP21 Antibody

• Background:

  TMP21 Antibody: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta; production, demonstrating that TMP21 can modulate gamma-secretase activity.

• NCBI Gene ID:

  10972

• Swiss Prot:

  P49755

• Accession Number:

  AAD31941

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  TMP21 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1403 - Mouse Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rabbit: (94%)

• Buffer:

  TMP21 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TMP21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  TMED10

• Positive Control 2:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Positive Control 3:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  Transmembrane emp24-like trafficking protein 10 (yeast)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Blunt R, Feick P, Puype M, et al. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem.1996; 271:17183-9.

• Background Reference 02:

  Chen F, Hasegawa H, Schmitt-Ulms G, et al. TMP21 is a presenilin complex component that modulates γ -secretase but not ε -secretase activity. Nature 2006; 440:1208-12.

• Background Reference 03:

  Periz G and Fortini ME. Functional reconstitution of γ -secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.

• Background Reference 04:

  Selkoe DJ. The cell biology of β -amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.

• Other Product Names:

  TMP21 Antibody: p23, TMP21, S31I125, Tmp-21-I, S31III125, P24 (DELTA), Transmembrane emp24 domain-containing protein 10, 21 kDa transmembrane-trafficking protein

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  4885697

• Physical Properties:

  Liquid