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TIP47 Antibody

CAT: 0223-3883-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3883-01Size:0.02 mg
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Background
TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
NCBI Gene ID
10226
Swiss Prot
O60664
Accession Number
O60664
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
3883
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar TIP47 expression profile in human cell lines detected by two independent anti-TIP47 antibodies that recognize different epitopes, 3883 against N-terminus domain and 3881 against C-terminus domain. TIP47 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3883 3881 Induced Expression Validation (Figure 6-9) : TIP47 expression detected by anit-TIP47 antibodies was increased in Atg7 liver KO mice (figure 6), after starvation (figure 7), after training (figure 8) or after oleic acid treatment (figure 9) . Induced Expression Validation
Field of Research
Signal Transduction
Purification
TIP47 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1287 - EL4 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Pig: (86%), Rat (79%)
Buffer
TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
TIP47 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human TIP47 has 4 isoforms, including isoform 1 (434aa, 47kD), isoform 2 (251aa, 28kD), isoform 3 (433aa, 47kD), isoform 4 (422aa, 46kD) . Mouse TIP47 has one isoform (437aa, 47kD) and rat TIP47 also has one isoform (438aa, 47kD) . 3883 can detect human isoforms 1, 3 and 4, and can also detect mouse and rat TIP47.
Symbol
PLIN3
Positive Control 2
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 3
Cat. No.1211 - HepG2 Cell Lysate
Positive Control 4
Cat. No. 1282 – 3T3/NIH Cell Lysate
NCBI Official Name
Perilipin 3
NCBI Organism
Homo sapiens
Background Reference 01
Barbero et al. J. Cell Biol. 2002; 156:511-8.
Background Reference 02
Carroll et al. Science 2001; 292:1373-7.
Background Reference 03
Aivazian et al. J. Cell Biol. 2006; 173:917-26.
Background Reference 04
Ganley et al. Mol. Biol. Cell 2004; 15:5420-30.
Citation 01
19339967
Citation 02
25663294
Citation 03
23603907
Citation 04
26143378
Citation 05
27386161
Citation 06
26471304
Citation 07
22667335
Citation 08
25552350
Citation 09
24303154
Citation 10
25318747
Other Product Names
TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein
Tested Applications
ELISA, WB, IHC-P
Protein ID
68846601
Physical Properties
Liquid

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