TIP47 Antibody

• Background:

  TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.

• NCBI Gene ID:

  10226

• Swiss Prot:

  O60664

• Accession Number:

  O60664

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3881

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar TIP47 expression profile in human cell lines detected by two independent anti-TIP47 antibodies that recognize different epitopes, 3883 against N-terminus domain and 3881 against C-terminus domain. TIP47 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3883 3881 Induced Expression Validation (Figure 6-8) : TIP47 expression detected by anit-TIP47 antibodies was increased in Atg7 liver KO mice (figure 6), after starvation (figure 7), or after oleic acid treatment (figure 8) . Induced Expression Validation

• Field of Research:

  Signal Transduction, Neuroscience

• Purification:

  TIP47 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (86%)

• Buffer:

  TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TIP47 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human TIP47 has 4 isoforms, including isoform 1 (434aa, 47kD), isoform 2 (251aa, 28kD), isoform 3 (433aa, 47kD), isoform 4 (422aa, 46kD) . Mouse TIP47 has one isoform (437aa, 47kD) and rat TIP47 also has one isoform (438aa, 47kD) . 3883 can detect all human isoforms, and can also detect mouse and rat TIP47.

• Symbol:

  PLIN3

• Positive Control 2:

  Cat. No. 1464 - Rat Liver Tissue Lysate

• Positive Control 3:

  Cat. No.1204 - K562 Cell Lysate

• NCBI Official Name:

  Perilipin 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Barbero et al. J. Cell Biol. 2002; 156:511-8.

• Background Reference 02:

  Carroll et al. Science 2001; 292:1373-7.

• Background Reference 03:

  Aivazian et al. J. Cell Biol. 2006; 173:917-26.

• Background Reference 04:

  Ganley et al. Mol. Biol. Cell 2004; 15:5420-30.

• Citation 01:

  19339967

• Citation 02:

  23603907

• Other Product Names:

  TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  68846601

• Physical Properties:

  Liquid