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IRE1p Antibody

CAT: 0223-3655-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3655-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI Gene ID
2081
Swiss Prot
O75460
Accession Number
O75460
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
3655
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KO validation (Figure 2) : Anti-IRE1p antibody (3655) specificity was further verified by IRE1P specific knockout. IRE1p signal was disrupted in IRE1p knockout HeLa cells in comparison with that in control HeLa cells. KO validation3655 Induced Expression Validation (Figure 11) : IRE1p expression detected by anit-IRE1p antibodies was up-regulated by 20 (S) ‐PPD treatment. Induced Expression Validation
Field of Research
Signal Transduction
Purification
IRE1p Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1288 - A20 Cell Lysate
Concentration
1 mg/mL
Buffer
IRE1p Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
IRE1p antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human IRE1p has 2 isoforms, including isoform 1 (977aa, 110kD) and isoform 2 (70aa, 6.6kD) . Mouse IRE1p also has two isoforms, including isoform 1 (977aa, 110kD) and isoform 2 (408aa, 45kD) . Rat IRE1p has one isoform (965aa, 109kD) . 3655 can detect human, mouse and rat.
Symbol
ERN1
Positive Control 2
Cat. No. 17-208 - A-20 Cell Slide
Positive Control 3
Cat. No. 1463 - Rat Brain Tissue Lysate
NCBI Official Name
Endoplasmic reticulum to nucleus signaling 1
NCBI Organism
Homo sapiens
Background Reference 01
Little et al. Crit. Rev. Eukaryot. Gene Expr. 1994;4:1-18.
Background Reference 02
Lee. Methods 2005;35:373-81.
Background Reference 03
Bertolotti et al. Nat. Cell Biol. 2000;2:326-32.
Background Reference 04
Shen et al. Dev. Cell 2002;3:99-111.
Citation 01
30259568
Other Product Names
IRE1p Antibody: IRE1, IRE1P, IRE1a, hIRE1p, IRE1, Endoplasmic reticulum-to-nucleus signaling 1
Tested Applications
ELISA, WB, ICC, IF, IHC-P
Protein ID
193806335
Physical Properties
Liquid

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