Bit1 Antibody

• Background:

  Bit1 Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

• NCBI Gene ID:

  51651

• Swiss Prot:

  Q9Y3E5

• Accession Number:

  NP_057161

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  Bit1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (94%)

• Buffer:

  Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Bit1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  PTRH2

• Positive Control 2:

  Cat. No. 1308 - Human Small Intestine Tissue Lysate

• Positive Control 3:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• NCBI Official Name:

  Peptidyl-tRNA hydrolase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.

• Background Reference 02:

  Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.

• Background Reference 03:

  Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.

• Other Product Names:

  Bit1 Antibody: BIT1, PTH2, CGI-147, BIT1, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1, PTH 2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  7706351

• Physical Properties:

  Liquid