ECSIT Antibody

• Background:

  ECSIT Antibody: Activation of NF-κ B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF) -6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF) -6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

• NCBI Gene ID:

  51295

• Swiss Prot:

  Q9BQ95

• Accession Number:

  NP_057665

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  ECSIT Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1301 - Human Heart Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  ECSIT Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  ECSIT antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  ECSIT

• Positive Control 2:

  Cat. No. 1401 - Mouse Heart Tissue Lysate

• NCBI Official Name:

  ECSIT homolog (Drosophila)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.

• Background Reference 02:

  Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.

• Background Reference 03:

  Janssens S and Beyaert R. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol. Cell. 2003; 11:293-302.

• Background Reference 04:

  Sato S, Sugiyama M, Yamamoto M, et al. Toll/IL-1 receptor domain-containing adaptor inducing IFN-β (TRIF) associates with TNF receptor-associated factor 6 and TANK-binding kinase 1, and activates two distinct transcription factors, NF-κ B and IFN-regulatory factor-3, in the Toll-like receptor signaling. J. Immunol. 2003; 171 (8) :4304-10.

• Other Product Names:

  ECSIT Antibody: SITPEC, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  20149633

• Physical Properties:

  Liquid