NADE Antibody

• Background:

  NADE Antibody: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF) . The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac) . Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

• NCBI Gene ID:

  27018

• Swiss Prot:

  Q00994

• Accession Number:

  NP_996798

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  NADE Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)

• Buffer:

  NADE Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NADE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  NGFRAP1

• Positive Control 2:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  Nerve growth factor receptor (TNFRSF16) associated protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39.

• Background Reference 02:

  Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70.

• Background Reference 03:

  Rapp G, Freudenstein J, Klaudiny J, et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 1990; 9:479-85.

• Background Reference 04:

  YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30.

• Other Product Names:

  NADE Antibody: Bex, BEX3, NADE, HGR74, DXS6984E, Protein BEX3, Brain-expressed X-linked protein 3

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  46094060

• Physical Properties:

  Liquid