Bcl-rambo Antibody

• Background:

  Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.

• NCBI Gene ID:

  23786

• Swiss Prot:

  Q9BXK5

• Accession Number:

  AAH07658

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  Bcl-rambo Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Bcl-rambo antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  BCL2L13

• Positive Control 2:

  Cat. No. 1301 - Human Heart Tissue Lysate

• Positive Control 3:

  Cat. No. 10-501 - Human Heart Tissue Slide

• NCBI Official Name:

  BCL2-like 13 (apoptosis facilitator)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

• Background Reference 02:

  Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.

• Background Reference 03:

  Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.

• Background Reference 04:

  Kataoka T, Holler N, Michau O, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J. Biol. Chem. 2001; 276:19548-54.

• Other Product Names:

  Bcl-rambo Antibody: MIL1, BCL-RAMBO, Bcl2-L-13, MIL1, CD003, Bcl-2-like protein 13, Bcl-rambo

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  14043326

• Physical Properties:

  Liquid