MTBP Antibody

• Background:

  MTBP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.

• NCBI Gene ID:

  27085

• Swiss Prot:

  Q96DY7

• Accession Number:

  NP_071328

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  MTBP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (94%)

• Buffer:

  MTBP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  MTBP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  MTBP

• Positive Control 2:

  Cat. No. 17-004 - K-562 Cell Slide

• NCBI Official Name:

  Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.

• Background Reference 02:

  Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.

• Background Reference 03:

  Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288 (5468) :1053-8.

• Background Reference 04:

  Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102 (6) :849-62.

• Other Product Names:

  MTBP Antibody: MDM2BP, Mdm2-binding protein, hMTBP

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  21630257

• Physical Properties:

  Liquid