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ASC Antibody

CAT: 0223-2287-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-2287-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4) . Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
NCBI Gene ID
29108
Swiss Prot
Q9ULZ3
Accession Number
BAA87339
Host
Rabbit
Reactivity
Human
Immunogen
2287
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar ASC expression profile in human and mouse cell lines detected by two independent anti-ASC antibodies that recognize different epitopes, 2287 against C-terminus domain and 39-001 against full length human recombinant protein. ASC proteins are detected in some of tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2287 39-001
Field of Research
Apoptosis, Inflammasomes
Purification
ASC Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1209 - HL60 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Mouse (57%), Rat (57%)
Buffer
ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
ASC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human ASC has 3 isoforms, including isoform 1 (195aa, 22kD), isoform 2 (176aa, 20kD), and isoform 3 (135aa, 15kD) . Mouse ASC has one isoform (193aa, 22kD) and rat ASC also has one isoform (193aa, 22kD) . 2287 can detect all human isoforms.
Symbol
PYCARD
Positive Control 2
Cat. No. 17-009 - HL-60 Cell Slide
Positive Control 3
Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 4
Cat. No. 1208 – THP-1 Cell Lysate
NCBI Official Name
PYD and CARD domain containing
NCBI Organism
Homo sapiens
Background Reference 01
Masumoto et al. J Biol Chem. 1999;274 (48) :33835-8.
Background Reference 02
Masumoto et al. Exp Cell Res. 2001;262 (2) :128-133.
Background Reference 03
Conway et al. Cancer Res. 2000;60 (22) :6236-42
Background Reference 04
McConnell et al. Cancer Res. 2000;60 (22) :6243-7. (WD0102)
Citation 01
15020601
Other Product Names
ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC
Tested Applications
ELISA, WB, ICC, IHC-P
Protein ID
10801602
Physical Properties
Liquid

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