FLASH Antibody

• Background:

  FLASH Antibody: A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein) . FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

• NCBI Gene ID:

  9994

• Swiss Prot:

  Q9UKL3

• Accession Number:

  AF165161

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  FLASH Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (94%)

• Buffer:

  FLASH Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  FLASH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  220 kDa

• Fragment:

  IgG

• Symbol:

  CASP8AP2

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Caspase 8 associated protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85

• Background Reference 02:

  Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3

• Background Reference 03:

  Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)

• Other Product Names:

  FLASH Antibody: CED-4, FLASH, RIP25, KIAA1315, CASP8-associated protein 2, FLICE-associated huge protein

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  5533376

• Physical Properties:

  Liquid