SkiP Antibody

• Background:

  SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.

• NCBI Gene ID:

  22938

• Swiss Prot:

  Q13573

• Accession Number:

  NP_036377

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  SkiP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  SkiP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SkiP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  SNW1

• Positive Control 2:

  Cat. No. 1305 - Human Kidney Tissue Lysate

• Positive Control 3:

  Cat. No. 10-401 - Human Kidney Tissue Slide

• NCBI Official Name:

  SNW domain containing 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.

• Background Reference 02:

  Li Y, Turck CM, Teumer JK, et al. Unique sequence, SkiP, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.

• Background Reference 03:

  Luo K. SkiP and SkiP: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.

• Background Reference 04:

  Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.

• Other Product Names:

  SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP

• Tested Applications:

  ELISA, WB, IF

• Protein ID:

  6912676

• Physical Properties:

  Liquid