BACE Antibody

• Background:

  BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) . BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

• NCBI Gene ID:

  23621

• Swiss Prot:

  P56817

• Accession Number:

  AF190725

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  2253

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar BACE expression profile in human cell lines detected by two independent anti-BACE antibodies that recognize different epitopes, 2253 against C-terminus domain and 32-238 against recombinant fragment protein. BACE proteins are detected in all the tested cell lines except K562 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2253 KO validation (Figure 6,9) : Anti-BACE antibody (2253) specificity was further verified by BACE KO mice (figure6) and KO cell line (figure9) . BACE signal was not detected in BACE KO mice and KO cell line. KO validation2253 KD validation (Figure 7,8,10) : Anti-BACE antibody (2253) specificity was verified by BACE specific siRNA knockdown. BACE signal in mouse brain injected with BACE siRNAs and DRG transfected with BACE siRNAs was disrupted in comparison with control. KD validation2253 Overexpression validation (Figure 6) : Anti-BACE antibody (2253) detected high expression levels of BACE in 293 cells transfected with hBACE or mBACE (figure6) as compared to eGFP transfected cells. Overexpression validation2253

• Field of Research:

  Neuroscience, Cancer

• Purification:

  BACE Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Guinea pig: (94%), Rat: (94%), Bovine: (94%)

• Buffer:

  BACE Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BACE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human BACE has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD) . This antibody detects all human isoforms. Mouse BACE has only 1 isoform (501aa, 55.7kD) . Rat BACE has only one isoform identified so far (501aa, 55.8kD) .

• Symbol:

  BACE1

• Positive Control 2:

  Cat. No. 1282 - 3T3/NIH Cell Lysate

• NCBI Official Name:

  Beta-site APP-cleaving enzyme 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Vassar et al. Science 1999;286:735-41

• Background Reference 02:

  Hussain et al. Mol Cell Neurosci 1999;14:419-27

• Background Reference 03:

  Yan et al. Nature 1999;402:533-7

• Background Reference 04:

  Sinha et al. Nature 1999;402:537-40

• Citation 01:

  16136043

• Citation 02:

  18687504

• Citation 04:

  20354142

• Citation 05:

  11863452

• Citation 06:

  19166938

• Citation 07:

  21073551

• Citation 08:

  14507929

• Citation 09:

  12480937

• Citation 10:

  16027115

• Citation 11:

  17912249

• Citation 12:

  18387811

• Citation 13:

  29217476

• Citation 14:

  18349519

• Citation 15:

  20026385

• Citation 16:

  17307738

• Citation 17:

  17877641

• Citation 18:

  20555139

• Citation 19:

  20005283

• Citation 20:

  22551668

• Other Product Names:

  BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2

• Tested Applications:

  ELISA, WB, ICC, IHC-P, IF

• Protein ID:

  6118538

• Physical Properties:

  Liquid