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BACE Antibody

CAT: 0223-2253-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-2253-01Size:0.02 mg
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Background
BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) . BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
NCBI Gene ID
23621
Swiss Prot
P56817
Accession Number
AF190725
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
2253
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar BACE expression profile in human cell lines detected by two independent anti-BACE antibodies that recognize different epitopes, 2253 against C-terminus domain and 32-238 against recombinant fragment protein. BACE proteins are detected in all the tested cell lines except K562 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2253 KO validation (Figure 6,9) : Anti-BACE antibody (2253) specificity was further verified by BACE KO mice (figure6) and KO cell line (figure9) . BACE signal was not detected in BACE KO mice and KO cell line. KO validation2253 KD validation (Figure 7,8,10) : Anti-BACE antibody (2253) specificity was verified by BACE specific siRNA knockdown. BACE signal in mouse brain injected with BACE siRNAs and DRG transfected with BACE siRNAs was disrupted in comparison with control. KD validation2253 Overexpression validation (Figure 6) : Anti-BACE antibody (2253) detected high expression levels of BACE in 293 cells transfected with hBACE or mBACE (figure6) as compared to eGFP transfected cells. Overexpression validation2253
Field of Research
Neuroscience, Cancer
Purification
BACE Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1303 - Human Brain Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Guinea pig: (94%), Rat: (94%), Bovine: (94%)
Buffer
BACE Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
BACE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human BACE has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD) . This antibody detects all human isoforms. Mouse BACE has only 1 isoform (501aa, 55.7kD) . Rat BACE has only one isoform identified so far (501aa, 55.8kD) .
Symbol
BACE1
Positive Control 2
Cat. No. 1282 - 3T3/NIH Cell Lysate
NCBI Official Name
Beta-site APP-cleaving enzyme 1
NCBI Organism
Homo sapiens
Background Reference 01
Vassar et al. Science 1999;286:735-41
Background Reference 02
Hussain et al. Mol Cell Neurosci 1999;14:419-27
Background Reference 03
Yan et al. Nature 1999;402:533-7
Background Reference 04
Sinha et al. Nature 1999;402:537-40
Citation 01
16136043
Citation 02
18687504
Citation 04
20354142
Citation 05
11863452
Citation 06
19166938
Citation 07
21073551
Citation 08
14507929
Citation 09
12480937
Citation 10
16027115
Citation 11
17912249
Citation 12
18387811
Citation 13
29217476
Citation 14
18349519
Citation 15
20026385
Citation 16
17307738
Citation 17
17877641
Citation 18
20555139
Citation 19
20005283
Citation 20
22551668
Other Product Names
BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2
Tested Applications
ELISA, WB, ICC, IHC-P, IF
Protein ID
6118538
Physical Properties
Liquid

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