DEDAF Antibody

• Background:

  DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

• NCBI Gene ID:

  23429

• Swiss Prot:

  Q8N488

• Accession Number:

  AF179286

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  DEDAF Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1203 - A549 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DEDAF antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  32 kDa

• Fragment:

  IgG

• Symbol:

  RYBP

• Positive Control 2:

  Cat. No. 1211 - HepG2 Cell Lysate

• Positive Control 3:

  Cat. No. 1404 - Mouse Liver Tissue Lysate

• Positive Control 4:

  Cat. No. 17-003 - A549 Cell Slide

• NCBI Official Name:

  RING1 and YY1 binding protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zheng L, Schickling O, Peter ME, et al. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J. Biol. Chem. 2001; 276:31945-52.

• Background Reference 02:

  Garcia E, Marcos-Gutierrez C, del Mar Lorente M, et al. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18:3404-18.

• Other Product Names:

  DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  5802963

• Physical Properties:

  Liquid