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DR4 Antibody

CAT: 0223-1167-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-1167-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) . The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) . DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κ B activation in many tissues and cells.
NCBI Gene ID
8797
Swiss Prot
O00220
Accession Number
AAC51226
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
1167
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KO Validation (Figure 1) : Anti-DR4 antibodies (1167) specificity was further verified by DR4 specific knockout cell lysate. DR4 signal was detected in wild type cells, but not in the DR4 knockout cells. KO Validation1167) Independent Antibody Validation in Cell lines (Figure 2) shows similar DR4 expression profile in human cell lines detected by two independent anti-DR4 antibodies that recognize different epitopes, 1139 against C-terminus domain and 1167 against the N-terminus domain. DR4 proteins are detected in all the tested cell lines except CaCo-2 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1139 1167 KD validation (Figure 6, 10) : Anti-DR4 antibody specificity was further verified by DR4 specific siRNA knockdown. DR4 signal in SW480 and HeLa cells transfected with DR4 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation
Field of Research
Apoptosis
Purification
DR4 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1202 - A431 Cell Lysate
Concentration
1 mg/ml
Buffer
DR4 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
DR4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
DR4 antibody has no cross reaction to DR5.
Isoform
Human DR4 has only 1 isoform (468aa, 50kD) .
Symbol
TNFRSF10A
Positive Control 2
Cat. No. 1203 - A549 Cell Lysate
Positive Control 3
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 4
Cat. No. 1201 - HeLa Cell Lysate
NCBI Official Name
Tumor necrosis factor receptor superfamily, member 10a
NCBI Organism
Homo sapiens
Background Reference 01
Pan et al. Science 1997;276:111-3
Background Reference 02
Wiley et al. Immunity 1995; 3:673-82
Background Reference 03
Pitti et al. J. Biol. Chem. 1996;271:12687-90
Background Reference 04
Schneider et al. Immunity 1997; 7:831-6
Citation 01
18193086
Citation 02
25032854
Citation 03
18511292
Citation 04
27827395
Citation 05
16007131
Citation 06
17470478
Citation 07
29020632
Citation 08
20562908
Citation 09
24980819
Citation 10
18594532
Citation 11
21941003
Citation 12
18599488
Citation 13
17212362
Citation 14
21835222
Citation 15
17522150
Citation 16
28529596
Citation 17
22784708
Citation 18
27406983
Citation 19
21372226
Citation 20
19414356
Other Product Names
DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1
Tested Applications
ELISA, WB, IF, ICC
Protein ID
1945072
Physical Properties
Liquid

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