DR4 Antibody

• Background:

  DR4 Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) . The ligand for this novel death receptor has been identified and termed TRAIL2, 3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) . DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-κ B activation in many tissues and cells.

• NCBI Gene ID:

  8797

• Swiss Prot:

  O00220

• Accession Number:

  AAC51226

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  1139

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 1) : Anti-DR4 antibodies (1139) specificity was further verified by DR4 specific knockout cell lysate. DR4 signal was detected in wild type cells, but not in the DR4 knockout cells. KO Validation1139) Independent Antibody Validation in Cell lines (Figure 2) shows similar DR4 expression profile in human cell lines detected by two independent anti-DR4 antibodies that recognize different epitopes, 1139 against C-terminus domain and 1167 against the N-terminus domain. DR4 proteins are detected in all the tested cell lines except CaCo-2 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines1139 1167 KD validation (Figure 7, 10, 11) : Anti-DR4 antibody (1139) specificity was further verified by DR4 specific siRNA knockdown. DR4 signal in SW480, Huh7 and HeLa cells transfected with DR4 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation1139

• Field of Research:

  Apoptosis

• Purification:

  DR4 Antibody is Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1202 - A431 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  DR4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DR4 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  DR4 antibody has no cross reaction to DR5

• Isoform:

  Human DR4 has only 1 isoform (468aa, 50kD) .

• Symbol:

  TNFRSF10A

• Positive Control 2:

  Cat. No. 1203 - A549 Cell Lysate

• Positive Control 3:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 4:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Tumor necrosis factor receptor superfamily, member 10a

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pan et al. Science 1997;276:111-3

• Background Reference 02:

  Wiley et al. Immunity 1995; 3:673-82

• Background Reference 03:

  Pitti et al. J. Biol. Chem. 1996;271:12687-90

• Background Reference 04:

  Schneider et al. Immunity 1997; 7:831-6

• Citation 01:

  18193086

• Citation 02:

  25032854

• Citation 03:

  18511292

• Citation 04:

  17470478

• Citation 05:

  16007131

• Citation 06:

  29020632

• Citation 07:

  20562908

• Citation 08:

  24980819

• Citation 09:

  18594532

• Citation 10:

  21941003

• Citation 11:

  18599488

• Citation 12:

  17212362

• Citation 13:

  21835222

• Citation 14:

  17522150

• Citation 15:

  28529596

• Citation 16:

  22784708

• Citation 17:

  27406983

• Citation 18:

  21372226

• Citation 19:

  19414356

• Citation 20:

  18680719

• Other Product Names:

  DR4 Antibody: DR4, APO2, CD261, TRAILR1, TRAILR-1, DR4, Tumor necrosis factor receptor superfamily member 10A, Death receptor 4, TRAIL receptor 1

• Tested Applications:

  ELISA, WB, IHC-P, ICC, IF

• Protein ID:

  1945072

• Physical Properties:

  Liquid