NIK Antibody

• Background:

  NIK Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα , LPS and mitogens. A serine/threonine protein kinase which mediates NF-κ B activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κ B inducing kinase) . NIK is an activator of Iκ B kinase alpha and beta (IKKα and IKKβ ) . Therefore, NIK is a key molecule in the NF-κ B signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.

• NCBI Gene ID:

  9020

• Swiss Prot:

  Q99558

• Accession Number:

  Q99558

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  NIK Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - HEK293 Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (94%)

• Buffer:

  NIK Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NIK antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  Predicted: 104 kDa

• Fragment:

  IgG

• Symbol:

  MAP3K14

• NCBI Official Name:

  Mitogen-activated protein kinase kinase kinase 14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κ B induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4.

• Background Reference 02:

  Regnier CH, Song HY, Gao X, et al. Identification and characterization of an Iκ B kinase. Cell 1997; 90:373-83.

• Background Reference 03:

  Woronicz JD, Gao X, Cao Z, et al. Iκ B kinase-β : NF-κ B activation and complex formation with Iκ B kinase-α and NIK. Science 1997; 278:866-9.

• Background Reference 04:

  Ling L, Cao Z, and Goeddel D. NF-κ B-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. USA 1998; 95:3792-7.

• Other Product Names:

  NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK

• Tested Applications:

  ELISA, WB, IF, ICC

• Protein ID:

  92090612

• Physical Properties:

  Liquid