Gfer (NM_023040) Mouse Recombinant Protein

• Background:

  FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Alr; ERV1

• Gene ID:

  11692

• UniProt:

  P56213

• Accession Number mRNA:

  NM_023040

• Chromosomal Location:

  17 12.48 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  22.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Gfer

• Species:

  Mouse

• Protein ID:

  NP_075527

• Overview:

  Purified recombinant protein of Mouse growth factor, augmenter of liver regeneration (Gfer), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/11692

• Uniprot URL:

  https://www.uniprot.org/uniprot/P56213

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_023040

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_075527