IL21 Protein, Mouse, Recombinant

• Background:

  IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as a therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation) .

• Synonyms:

  IL-21 Protein, Mouse

• Gene Name:

  Interleukin 21

• UniProt:

  Q9ES17

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The EDfor this effect is typically 4-20 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins, Immunotherapy proteins

• Molecular Weight:

  The recombinant mouse IL21 consists of 123 amino acids and has a calculated molecular mass of 14.3KDa. It migrates as a 15 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL21

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the soluble form of mouse IL21 (Q9ES17) (Pro 25-Ser 146) was expressed, with an initial Met at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9ES17

• AA Sequence:

  Pro25-Ser146