GIT1 (NM_014030) Human Recombinant Protein

• Background:

  GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  G protein-coupled receptor kinase-interactor 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; OTTHUMP00000163606

• Gene ID:

  28964

• UniProt:

  Q9Y2X7

• Accession Number mRNA:

  NM_014030

• Chromosomal Location:

  17q11.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton

• Field of Research:

  Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  84.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  GIT1

• Species:

  Human

• Protein ID:

  NP_054749

• Overview:

  Recombinant protein of human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/28964

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Y2X7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_014030

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_054749