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E2f1 (NM_007891) Mouse Recombinant Protein

CAT: 0882-PM46416M5Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0882-PM46416M5Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:11672531, PubMed:20176812) . Positively regulates transcription of RRP1B (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse E2F transcription factor 1 (E2f1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
E2F-1; mKIAA4009; Tg (Wnt1-cre) 2Sor
Gene ID
13555
UniProt
Q61501, Q547J6, Q9CYB4
Accession Number mRNA
NM_007891
Chromosomal Location
2 76.79 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
46.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
E2f1
Species
Mouse
Protein ID
NP_031917
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/13555
Uniprot URL
https://www.uniprot.org/uniprot/Q61501
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_007891
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_031917

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