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Papss1 (BC066055) Mouse Recombinant Protein

CAT: 0882-PM45527M5Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0882-PM45527M5Size:20 µg
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Background
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase) . In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984) . Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
AI325286; Asapk; SK1
Gene ID
23971
UniProt
Q60967
Accession Number mRNA
BC066055
Chromosomal Location
3 61.05 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
68.5 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Papss1
Species
Mouse
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/23971
Uniprot URL
https://www.uniprot.org/uniprot/Q60967
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/BC066055

UniProtKB · Q60967

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1

PAPS1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q60967
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Papss1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PAPS synthase 1, PAPSS 1
EC number
—
Processing
—
Secondary accessions
Q3U647
Protein keywords

PTM

Acetylation

Ligand

ATP-bindingNucleotide-binding

Molecular function

KinaseMultifunctional enzymeNucleotidyltransferaseTransferase

Technical term

Reference proteome

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