Ngef (NM_001111314) Mouse Recombinant Protein

• Background:

  Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Ephexin; Tims2

• Gene ID:

  53972

• UniProt:

  Q8CHT1, E9QK62

• Accession Number mRNA:

  NM_001111314

• Chromosomal Location:

  1 44.42 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  82.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ngef

• Species:

  Mouse

• Protein ID:

  NP_001104784

• Overview:

  Purified recombinant protein of Mouse neuronal guanine nucleotide exchange factor (Ngef), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/53972

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8CHT1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001111314

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001104784