Nthl1 (NM_008743) Mouse Recombinant Protein

• Background:

  Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Nth1; Octs3

• Gene ID:

  18207

• UniProt:

  O35980

• Accession Number mRNA:

  NM_008743

• Chromosomal Location:

  17 A3.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  33.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Nthl1

• Species:

  Mouse

• Protein ID:

  NP_032769

• Overview:

  Purified recombinant protein of Mouse nth (endonuclease III) -like 1 (E.coli) (Nthl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18207

• Uniprot URL:

  https://www.uniprot.org/uniprot/O35980

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008743

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032769