IL-1 beta Protein, Canine, Recombinant

• Background:

  Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra) . In clinical, it has been reported that Interleukin (IL) -1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of a successful pregnancy. Proinflammatory interleukin (IL) -1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc) .

• Gene Name:

  Interleukin 1, beta

• UniProt:

  Q28292

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Neuroinflammation

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 96 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its ability to bind human IL1R1 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The EDfor this effect is 0.2-1ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM PB, 0.3M NaCl, pH 6.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Diagnostic proteins, Immunotherapy proteins, Biomarker Proteins, Cell Therapy proteins, Biological Target proteins

• Molecular Weight:

  The recombinant canine IL1B consists of 153 amino acids and has a calculated molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL1B

• Species:

  Canine

• Overview:

  A DNA sequence encoding the mature form of canine IL1B (Q28292) (Ala 115-Ser 266) was expressed, with an initial Met at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q28292

• AA Sequence:

  Ala115-Ser266