IL-1 beta Protein, Rat, Recombinant (pro form, His Tag)

• Background:

  Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra) . In clinical, it has been reported that Interleukin (IL) -1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of a successful pregnancy. Proinflammatory interleukin (IL) -1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc) .

• Gene Name:

  Interleukin 1, beta

• UniProt:

  Q63264-1

• Expression System:

  E. coli

• Tag:

  N-His

• Field of Research:

  Neuroinflammation

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM Tris, 500mM NaCl, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Diagnostic proteins, Immunotherapy proteins, Cell Therapy proteins, Biological Target proteins, Biomarker Proteins

• Molecular Weight:

  The recombinant rat IL1B comprises 279 amino acids and predicts a molecular mass of 32.1 kDa. The apparent molecular mass of the rat IL1B is approximately 35 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL1B

• Species:

  Rat

• Overview:

  A DNA sequence encoding the pro form of rat IL1B (Q63264-1) (Met 1-Ser 268) was fused with a polyhistidine tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q63264-1

• AA Sequence:

  Met1-Ser268