SARS-CoV-2 (2019-nCoV) 3CLpro / 3C-like protease-His Recombinant Protein

• Background:

  3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab) . SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >5 pmols/min/μg

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, pH8.0, 300mM NaCl, 10% glycerol Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Viral Proteins, 2019-nCoV, HCoV

• Molecular Weight:

  The recombinant SARS-CoV-2 (2019-nCoV) 3CL Protease consists of 317 amino acids and predicts a molecular mass of 35.31 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  3CLpro

• Species:

  SARS-CoV-2

• Protein ID:

  YP_009725295.1

• Overview:

  A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CL Protease (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminal polyhistidine tagged.

• AA Sequence:

  Ser3264-Gln3569

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/YP_009725295.1