SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein

• Background:

  NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulate RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis.

• Expression System:

  E. coli

• Tag:

  C-AVI

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris 500mM NaCl pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Viral Proteins, 2019-nCoV, HCoV

• Molecular Weight:

  The recombinant SARS-CoV-2 (2019-nCoV) NSP8 consists of 220 amino acids and predicts a molecular mass of 24.17 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NSP8-CoV

• Species:

  SARS-CoV-2

• Protein ID:

  YP_009725304.1

• Overview:

  A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP8 Protein (YP_009725304.1) (Ala1-Gln198) was expressed with a AVI tag at the C-terminus.

• AA Sequence:

  Ala1-Gln198

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/YP_009725304.1