SARS-CoV-2 (2019-nCoV) NSP10-His Recombinant Protein

• Background:

  NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  HCoV, 2019-nCoV

• Molecular Weight:

  The recombinant SARS-CoV-2 (2019-nCoV) NSP10 Protein (His tag) consists of 146 amino acids and predicts a molecular mass of 15.7 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NSP10-CoV

• Species:

  SARS-CoV-2

• Protein ID:

  YP_009725306.1

• Overview:

  A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP10 (YP_009725306.1) (Ala1-Gln139) was expressed with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

• AA Sequence:

  Ala1-Gln139

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/YP_009725306.1