FGF9 Protein, Canine, Recombinant (mFc Tag)

• Background:

  Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

• Gene Name:

  Fibroblast growth factor 9

• Expression System:

  HEK293 Cells

• Tag:

  N-mFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 75 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The EDfor this effect is typically 5-30 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Growth Factor proteins, Biological Target proteins, Cell Therapy proteins, Cytokine Proteins

• Molecular Weight:

  The recombinant canine FGF9 comprises 441 amino acids and has a predicted molecular mass of 49.8 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF9

• Species:

  Canine

• Protein ID:

  NP_002001.1

• Overview:

  A DNA sequence encoding the canine FGF9 (NP_002001.1) (Leu4-Ser208) was expressed with the Fc region of mouse IgG1 at the N-terminus.

• AA Sequence:

  Leu4-Ser208

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002001.1