FGF14 Protein, Canine, Recombinant

• Background:

  FGF14 is a member of the fibroblast growth factor (FGF) family. Members of this family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF14 is probably involved in nervous system development and function. Defects in FGF14 are the cause of spinocerebellar ataxia type 27 (SCA27) . It is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA27 is an autosomal dominant cerebellar ataxia. It is a slowly progressive disorder, with onset in late-childhood to early adulthood, characterized by ataxia with tremor, orofacial dyskinesia, psychiatric symptoms and cognitive deficits.

• Gene Name:

  Fibroblast growth factor 14

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA. Immobilized Canine FGF14 (Cat:PH51626M3) at 2 μg/ml (100 μl/well) can bind Human FGFR4 hFc (Cat:10538-h02h), the ECof Human FGFR4 hFc is 300-950 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Growth Factor proteins, Cytokine Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant canine FGF14 comprises 190 amino acids and has a predicted molecular mass of 21.1 kDa. The apparent molecular mass of the protein is approximately 21 kDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF14

• Species:

  Canine

• Protein ID:

  XP_003363267.1

• Overview:

  A DNA sequence encoding the canine FGF14 (XP_003363267.1) (Lys64-Thr252) was expressed with a N-terminal Met.

• AA Sequence:

  Lys64-Thr252

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/XP_003363267.1